Acitivity of yeast extracts in cell-free stimulation of DNA replication.
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چکیده
منابع مشابه
Activity of yeast extracts in cell - free stimulation of DNA replication ( DNA synthesis in vitro / growth control / cell cycle / cdc mutants )
Extracts of the cytoplasm of disrupted spheroplasts of Saccharomyces cerevisiae (bakers' yeast) stimulated DNA synthesis in a cell-free system consisting of nuclei from spleen cells of the frog Xenopus laevis. The stimulation required Mg++, ATP, and the deoxynucleoside triphosphates, was saturated by an excess of nuclei or extract, and had kinetics resembling those obtained previously with extr...
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15 صفحه اولDNA synthesis in yeast cell-free extracts dependent on recombinant DNA plasmids purified from Escherichia coli.
In our attempts to establish a cell-free DNA replication system for the yeast Saccharomyces cerevisiae, we have observed that recombinant DNA plasmids purified from Escherichia coli by a common procedure (lysozyme-detergent lysis and equilibrium banding in cesium chloride ethidium bromide gradients) often serve as templates for DNA synthesis by elongation enzymes. The templates could be elongat...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1976
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.73.11.3933